Radical S-Adenosylmethionine Enzyme Coproporphyrinogen III Oxidase HemN

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Crystal structure of coproporphyrinogen III oxidase reveals cofactor geometry of Radical SAM enzymes.

'Radical SAM' enzymes generate catalytic radicals by combining a 4Fe-4S cluster and S-adenosylmethionine (SAM) in close proximity. We present the first crystal structure of a Radical SAM enzyme, that of HemN, the Escherichia coli oxygen-independent coproporphyrinogen III oxidase, at 2.07 A resolution. HemN catalyzes the essential conversion of coproporphyrinogen III to protoporphyrinogen IX dur...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 2005

ISSN: 0021-9258

DOI: 10.1074/jbc.m501275200